Test - lowercase jpg
Brand AVX TPSE226M035R0125 Low impedance tantalum capacitor AVX 22
Electronic scale crystal oscillator 3.2*2.5mm 3225 16M (16.000MHZ) 12PF 10PPM 20PPM 30PPM
Kaixin micro test
Test probe P100-M3
1. Standard Dilution: This kit includes one original standard. You can dilute it in a small tube according to the provided chart. It's important to follow the dilution ratio carefully to ensure accurate results.
2. Sample Addition: Prepare blank wells, standard wells, and sample wells separately. For the blank control, do not add the sample or enzyme standard reagent, but follow all other steps. Add 50 μl of the standard to the enzyme-labeled plate, then 40 μl of the sample diluent into the well you're testing, followed by 10 μl of the sample itself. This gives a final 5x dilution. Be careful to add the sample to the bottom of the well without touching the sides, and mix gently after adding.
3. Incubation: Seal the plate with an adhesive film and incubate at 37°C for 30 minutes. Make sure the environment is stable during this time.
4. Washing Solution Preparation: Dilute the 30x concentrated washing solution with distilled water at a 1:30 ratio before use.
5. Washing: Remove the sealing film, discard the liquid, and dry the plate. Fill each well with the washing solution, let it sit for 30 seconds, then discard. Repeat this process five times, and pat the plate dry thoroughly.
6. Enzyme Reagent Addition: Add 50 μl of the enzyme-labeled reagent to each well except the blank ones. Ensure even distribution across all wells.
7. Incubation: Repeat the same incubation step as in point 3—seal the plate and incubate at 37°C for 30 minutes.
8. Washing: Perform the washing procedure again as described in step 5, ensuring all residual liquids are removed.
9. Color Development: Add 50 μl of color developer A, followed by 50 μl of color developer B. Gently shake the plate to mix the contents. Allow the reaction to develop for 15 minutes at 37°C.
10. Stop Reaction: Add 50 μl of stop solution to each well to halt the reaction. The color will change from blue to yellow.
11. Measurement: Measure the absorbance (OD value) of each well using a spectrophotometer. Set the blank well to zero and measure at a wavelength of 450 nm. Perform the measurement within 15 minutes after adding the stop solution to ensure accuracy.
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