Rat platelet activating factor kit instruction manual - Huaqiang Electronic Network

Test - lowercase jpg
Brand AVX TPSE226M035R0125 Low impedance tantalum capacitor AVX 22
Electronic scale crystal oscillator 3.2*2.5mm 3225 16M (16.000MHZ) 12PF 10PPM 20PPM 30PPM
Kaixin micro test
Test probe P100-M3
Steps
1. Dilution of standard: This kit includes one original standard, which can be diluted in a small tube based on the provided chart. Make sure to follow the dilution ratios carefully to ensure accurate results. 2. Adding samples: Set up blank control wells, standard wells, and sample wells separately. In blank wells, do not add the sample or enzyme standard reagent, but follow all other steps. Add 50 μl of standard to the enzyme-labeled plate, then add 40 μl of sample diluent to the well being tested, followed by 10 μl of the sample. This results in a final 5-fold dilution. Add the sample to the bottom of the well, avoiding contact with the walls, and gently mix to ensure even distribution. 3. Incubation: Seal the plate with a sealing film and incubate at 37°C for 30 minutes. Ensure that the temperature remains stable throughout this period. 4. Prepare wash solution: Dilute the 30x concentrated washing solution with distilled water in a 1:30 ratio before use. 5. Washing: Remove the sealing film, discard the liquid, and dry the plate. Fill each well with the washing solution, let it sit for 30 seconds, then discard. Repeat this process five times, and pat the plate dry thoroughly. 6. Add enzyme reagent: Add 50 μl of the enzyme-labeled reagent to each well, except for the blank wells. Be careful not to introduce any contamination during this step. 7. Incubation: Repeat the same incubation procedure as step 3—seal the plate and incubate at 37°C for 30 minutes. 8. Washing: Perform the same washing steps as in step 5 to remove any unbound reagents. 9. Color development: Add 50 μl of color developer A to each well, followed by 50 μl of color developer B. Gently shake the plate to mix the solutions and allow the reaction to develop for 15 minutes at 37°C. 10. Stop reaction: Add 50 μl of stop solution to each well to terminate the color development. The blue color will turn yellow once the reaction stops. 11. Measurement: Measure the absorbance (OD value) of each well using a microplate reader. Set the blank well to zero and measure at a wavelength of 450 nm. Complete the measurement within 15 minutes after adding the stop solution to ensure accuracy.

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